claudin2  (Cell Signaling Technology Inc)

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Expression and localisation of Claudin2 in ISKNV-infected tissues. A Expression of three Claudin genes in the spleen from ISKNV-infected S. chuatsi analysed by qPCR. The data shown are representative of three experiments and presented as means ± SD of three detections. * p < 0.05, ** p < 0.01 by one-way ANOVA with Dunnett's post hoc test. B Immunofluorescence analysis of Claudin2 (green) in spleen tissues of S. chuatsi at 0, 1-, 2-, 3-, 4-, and 5-days post-ISKNV infection. The cytoplasm and the nucleus were marked by anti-MCP antibody (red) and Hoechst 33,258 (blue). C Claudin2 (green) was present on the surface of ISKNV-infected cells marked by anti-VP23R antibody (red) in S. chuatsi spleen tissues at 5 dpi. White arrows indicate the fluorescence signals of Claudin2 in LECs attaching on infected cells.

Article Snippet: The in vivo interaction was analysed in lysed spleen tissues of ISKNV-infected S. chuatsi at 5 dpi using Protein A/G plus agarose (Cat#sc-2003, Santa Cruz, USA) and the rabbit antibody against S. chuatsi Claudin2 (customised from GL Biochem China).

Techniques: Expressing, Infection, Immunofluorescence, Fluorescence

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Interaction of Claudin2 with VP08R. A Co-IP and reciprocal Co-IP analysed the interaction between Claudin2 and VP08R in transfected HEK293T cells. B Co-IP analysed the interaction between Claudin2 and VP08R in ISKNV-infected spleen tissues. C Diagram of the Claudin2 fragments. D The interaction of VP08R with the fragments of Claudin2 analysed by Co-IP. E Diagram of the VP08R fragments. F, G The interactions of VP08R fragments with the 35–81 aa ( F ) and 140–163 aa ( G ) regions of Claudin2 analysed by Co-IP.

Article Snippet: The in vivo interaction was analysed in lysed spleen tissues of ISKNV-infected S. chuatsi at 5 dpi using Protein A/G plus agarose (Cat#sc-2003, Santa Cruz, USA) and the rabbit antibody against S. chuatsi Claudin2 (customised from GL Biochem China).

Techniques: Co-Immunoprecipitation Assay, Transfection, Infection

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Interaction of Claudin2 with VP23R. A Co-IP and reciprocal Co-IP analyses of the interaction between Claudin2 and VP23R in vitro. B Co-IP analysis of the interaction between Claudin2 and VP23R in vivo. C Diagram of the VP23R fragments. D Co-IP analysis of the interaction of VP23R with the fragments of Claudin2. E Co-IP analysis of the interaction of VP23R fragments with the 35–81 aa region of Claudin2.

Article Snippet: The in vivo interaction was analysed in lysed spleen tissues of ISKNV-infected S. chuatsi at 5 dpi using Protein A/G plus agarose (Cat#sc-2003, Santa Cruz, USA) and the rabbit antibody against S. chuatsi Claudin2 (customised from GL Biochem China).

Techniques: Co-Immunoprecipitation Assay, In Vitro, In Vivo

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Interaction between Claudin2 and VP33L. A Co-IP and reciprocal Co-IP analyses of the Claudin2-VP33L interaction. B Pulldown assay of the interaction between GFP-tagged Claudin2 and ISKNV-encoded VP33L in MFF-1 cells. C Diagram of the VP33L fragments. D Co-IP analysis of the interaction of VP33L with Claudin2 fragments. E Co-IP analysis of the interaction of Claudin2 with VP33L fragments. F No interaction was observed between Claudin2 and nidogen.

Article Snippet: The in vivo interaction was analysed in lysed spleen tissues of ISKNV-infected S. chuatsi at 5 dpi using Protein A/G plus agarose (Cat#sc-2003, Santa Cruz, USA) and the rabbit antibody against S. chuatsi Claudin2 (customised from GL Biochem China).

Techniques: Co-Immunoprecipitation Assay

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Diagram of the interaction between Claudin2 and VMBM components. A Interactions between the extracellular loop regions of Claudin2 and VMBM components. Two-way arrows indicate the interaction between each other. B The structure of VMBM and its interaction with LECs. In addition to the proteins labelled in the diagram, the blue dumbbell-shaped structure indicates the nidogen protein.

Article Snippet: The in vivo interaction was analysed in lysed spleen tissues of ISKNV-infected S. chuatsi at 5 dpi using Protein A/G plus agarose (Cat#sc-2003, Santa Cruz, USA) and the rabbit antibody against S. chuatsi Claudin2 (customised from GL Biochem China).

Techniques:

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Expression and localisation of Claudin2 in ISKNV-infected tissues. A Expression of three Claudin genes in the spleen from ISKNV-infected S. chuatsi analysed by qPCR. The data shown are representative of three experiments and presented as means ± SD of three detections. * p < 0.05, ** p < 0.01 by one-way ANOVA with Dunnett's post hoc test. B Immunofluorescence analysis of Claudin2 (green) in spleen tissues of S. chuatsi at 0, 1-, 2-, 3-, 4-, and 5-days post-ISKNV infection. The cytoplasm and the nucleus were marked by anti-MCP antibody (red) and Hoechst 33,258 (blue). C Claudin2 (green) was present on the surface of ISKNV-infected cells marked by anti-VP23R antibody (red) in S. chuatsi spleen tissues at 5 dpi. White arrows indicate the fluorescence signals of Claudin2 in LECs attaching on infected cells.

Article Snippet: The coding sequences of GFP (as control) and GFP-tagged Claudin2 were cloned into the pcDNA3.1A vector (Invitrogen, USA) and transfected into MFF-1 cells.

Techniques: Expressing, Infection, Immunofluorescence, Fluorescence

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Interaction of Claudin2 with VP08R. A Co-IP and reciprocal Co-IP analysed the interaction between Claudin2 and VP08R in transfected HEK293T cells. B Co-IP analysed the interaction between Claudin2 and VP08R in ISKNV-infected spleen tissues. C Diagram of the Claudin2 fragments. D The interaction of VP08R with the fragments of Claudin2 analysed by Co-IP. E Diagram of the VP08R fragments. F, G The interactions of VP08R fragments with the 35–81 aa ( F ) and 140–163 aa ( G ) regions of Claudin2 analysed by Co-IP.

Article Snippet: The coding sequences of GFP (as control) and GFP-tagged Claudin2 were cloned into the pcDNA3.1A vector (Invitrogen, USA) and transfected into MFF-1 cells.

Techniques: Co-Immunoprecipitation Assay, Transfection, Infection

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Interaction of Claudin2 with VP23R. A Co-IP and reciprocal Co-IP analyses of the interaction between Claudin2 and VP23R in vitro. B Co-IP analysis of the interaction between Claudin2 and VP23R in vivo. C Diagram of the VP23R fragments. D Co-IP analysis of the interaction of VP23R with the fragments of Claudin2. E Co-IP analysis of the interaction of VP23R fragments with the 35–81 aa region of Claudin2.

Article Snippet: The coding sequences of GFP (as control) and GFP-tagged Claudin2 were cloned into the pcDNA3.1A vector (Invitrogen, USA) and transfected into MFF-1 cells.

Techniques: Co-Immunoprecipitation Assay, In Vitro, In Vivo

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Interaction between Claudin2 and VP33L. A Co-IP and reciprocal Co-IP analyses of the Claudin2-VP33L interaction. B Pulldown assay of the interaction between GFP-tagged Claudin2 and ISKNV-encoded VP33L in MFF-1 cells. C Diagram of the VP33L fragments. D Co-IP analysis of the interaction of VP33L with Claudin2 fragments. E Co-IP analysis of the interaction of Claudin2 with VP33L fragments. F No interaction was observed between Claudin2 and nidogen.

Article Snippet: The coding sequences of GFP (as control) and GFP-tagged Claudin2 were cloned into the pcDNA3.1A vector (Invitrogen, USA) and transfected into MFF-1 cells.

Techniques: Co-Immunoprecipitation Assay

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Diagram of the interaction between Claudin2 and VMBM components. A Interactions between the extracellular loop regions of Claudin2 and VMBM components. Two-way arrows indicate the interaction between each other. B The structure of VMBM and its interaction with LECs. In addition to the proteins labelled in the diagram, the blue dumbbell-shaped structure indicates the nidogen protein.

Article Snippet: The coding sequences of GFP (as control) and GFP-tagged Claudin2 were cloned into the pcDNA3.1A vector (Invitrogen, USA) and transfected into MFF-1 cells.

Techniques:

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Expression and localisation of Claudin2 in ISKNV-infected tissues. A Expression of three Claudin genes in the spleen from ISKNV-infected S. chuatsi analysed by qPCR. The data shown are representative of three experiments and presented as means ± SD of three detections. * p < 0.05, ** p < 0.01 by one-way ANOVA with Dunnett's post hoc test. B Immunofluorescence analysis of Claudin2 (green) in spleen tissues of S. chuatsi at 0, 1-, 2-, 3-, 4-, and 5-days post-ISKNV infection. The cytoplasm and the nucleus were marked by anti-MCP antibody (red) and Hoechst 33,258 (blue). C Claudin2 (green) was present on the surface of ISKNV-infected cells marked by anti-VP23R antibody (red) in S. chuatsi spleen tissues at 5 dpi. White arrows indicate the fluorescence signals of Claudin2 in LECs attaching on infected cells.

Article Snippet: They were then incubated with a rabbit antibody against Claudin2 (customised from GL Biochem, China) and a mouse antibody against MCP or VP23R [ ].

Techniques: Expressing, Infection, Immunofluorescence, Fluorescence

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Interaction of Claudin2 with VP08R. A Co-IP and reciprocal Co-IP analysed the interaction between Claudin2 and VP08R in transfected HEK293T cells. B Co-IP analysed the interaction between Claudin2 and VP08R in ISKNV-infected spleen tissues. C Diagram of the Claudin2 fragments. D The interaction of VP08R with the fragments of Claudin2 analysed by Co-IP. E Diagram of the VP08R fragments. F, G The interactions of VP08R fragments with the 35–81 aa ( F ) and 140–163 aa ( G ) regions of Claudin2 analysed by Co-IP.

Article Snippet: They were then incubated with a rabbit antibody against Claudin2 (customised from GL Biochem, China) and a mouse antibody against MCP or VP23R [ ].

Techniques: Co-Immunoprecipitation Assay, Transfection, Infection

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Interaction of Claudin2 with VP23R. A Co-IP and reciprocal Co-IP analyses of the interaction between Claudin2 and VP23R in vitro. B Co-IP analysis of the interaction between Claudin2 and VP23R in vivo. C Diagram of the VP23R fragments. D Co-IP analysis of the interaction of VP23R with the fragments of Claudin2. E Co-IP analysis of the interaction of VP23R fragments with the 35–81 aa region of Claudin2.

Article Snippet: They were then incubated with a rabbit antibody against Claudin2 (customised from GL Biochem, China) and a mouse antibody against MCP or VP23R [ ].

Techniques: Co-Immunoprecipitation Assay, In Vitro, In Vivo

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Interaction between Claudin2 and VP33L. A Co-IP and reciprocal Co-IP analyses of the Claudin2-VP33L interaction. B Pulldown assay of the interaction between GFP-tagged Claudin2 and ISKNV-encoded VP33L in MFF-1 cells. C Diagram of the VP33L fragments. D Co-IP analysis of the interaction of VP33L with Claudin2 fragments. E Co-IP analysis of the interaction of Claudin2 with VP33L fragments. F No interaction was observed between Claudin2 and nidogen.

Article Snippet: They were then incubated with a rabbit antibody against Claudin2 (customised from GL Biochem, China) and a mouse antibody against MCP or VP23R [ ].

Techniques: Co-Immunoprecipitation Assay

Journal: Veterinary Research

Article Title: Claudin2 is involved in the interaction between Megalocytivirus -induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

doi: 10.1186/s13567-024-01404-9

Figure Lengend Snippet: Diagram of the interaction between Claudin2 and VMBM components. A Interactions between the extracellular loop regions of Claudin2 and VMBM components. Two-way arrows indicate the interaction between each other. B The structure of VMBM and its interaction with LECs. In addition to the proteins labelled in the diagram, the blue dumbbell-shaped structure indicates the nidogen protein.

Article Snippet: They were then incubated with a rabbit antibody against Claudin2 (customised from GL Biochem, China) and a mouse antibody against MCP or VP23R [ ].

Techniques:

Journal: Microorganisms

Article Title: Lacticaseibacillus casei IB1 Alleviates DSS-Induced Inflammatory Bowel Disease by Regulating the Microbiota and Restoring the Intestinal Epithelial Barrier

doi: 10.3390/microorganisms12071379

Figure Lengend Snippet: L. casei IB1 upregulated tight junction proteins to improve colonic barrier dysfunction in DSS-induced colitis mice. ( A – C ) Statistical analysis of Occludin, Claudin1, and Claudin2 levels in the colon. The data are shown as the means ± SEMs. # p < 0.05, ## p < 0.01, compared to the CON group. * p < 0.05, ** p < 0.01, compared to the DSS group.

Article Snippet: After that, the PVDF membranes were incubated with the primary antibodies overnight at 4 °C: NF-κBP65 (8242S, CST, 1:1000), NF-κBP-p65 (3033S, CST, 1:1000), P38 (8690S, CST, 1:1000), P-p38 (4511S, CST, 1:1000), ERK1/2 (4695S, CST, 1:1000), P-ERK1/2 (4370S, CST, 1:1000), JNK (67096S, CST, 1:1000), P-JNK (4668S, CST, 1:1000), Occludin (27260, Proteintech, Rosemont, IL, USA, 1:1000), Claudin1 (37–4900, Invitrogen, Waltham, MA, USA), Claudin2 (AF0128, Affbiotech, Cincinnati, OH, USA, 1:1000), and β-Actin (HC201, TransGen Biotech, Beijing, China, 1:1000).

Techniques: